Yes.  With QBO and Transaction Pro, there is a separate subscription required to connect to a QBO file.  This is different than with QuickBooks Desktop, where a single TPI subscription could be connected to multiple companies.
The subscription with Transaction Pro is also monthly.

If we have a large clean up for a client and need TPI just for the clean up, we purchase the subscription for a single month and then cancel.  There is no annual requirement for the subscription, it is month to month.

Learn more about Transaction Pro Importer.

Updated: April2017